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Equipment / Instruments

The UW–Madison Keck Center for Chemical Genomics is located on the 5th floor of the Chemistry Department (Rm 5201, 5207A) and houses considerable modern chemical synthesis and biological instrumentation. Some equipment is located outside of the 5th floor, see locations and descriptions below.

 

Equipment / Instruments

CEM Microwave (Discover + Explorer)
Location: Rm 5201
Microwave assisted chemistry can be used in a variety of applications including reactions such as Heck and Suzuki couplings, challenging substitutions, Diels-Alder reactions, as well as peptide or polymer synthesis. Perform reactions at temperatures up to 300 °C in 5 minutes or less, with automation allowing up to 24 reactions to be queued and completed one-at-a-time. Contact Keck Center TA for training.

Perkin-Elmer Envision plate reader
Location: Rm 5201
The instrument includes two detectors enabling simultaneous dual wavelength reading, below emission reading, barcode readers, a high-speed light source and adjustment of measurement height function. Interchangeable mirrors and filters allow for less noise in absorption and emission readings. The plate reader can perform fast kinetic measurements, enzyme assays and numerous other cell-based assays. Example uses include: absorption, fluorescence, luminescence, fluorescence polarization (FP), and FRET measurements. Contact Keck Center TA for training.

ThermoOriel solar simulator
Location: Rm 5201
The solar simulator uses a xenon arc lamp to produce a continuous, uniform, collimated beam of radiation that closely matches the solar spectrum. Beam collimation simulates the direct terrestrial beam and allows for characterization of radiation-induced phenomena. Contact Keck Center TA for training. Currently Out Of Order.

Baker SterilGard II biosafety cabinet (BSC)
Location: Rm 5207A
This biosafety cabinet enables users to work with BSL 1 or 2 materials safely by providing three types of protection: (1) personnel protection from harmful agents inside the cabinet, (2) product protection to avoid contamination of the work, experiment, or process, and (3) environmental protection from contaminants contained within the cabinet. The proper sterilization of the BSC, before and after use, is paramount to preventing contamination of all users samples. Currently groups use this BSC for both prokaryotic and eukaryotic biology, and all users must have a biological safety protocol on file with the Office of Biological Safety. (OBS website) Contact Keck Center TA for training.

ThermoFisher Barnstead Nanopure water (18MΩ)
Location: Rm 6143
Ultrapure water is used for sensitive analytical procedures including atomic absorption, ICP and ICP/MS, IC, GC and high-wavelength HPLC, as well as many sensitive microbiology applications. This unit produces up to 1.5 L/min of Type I water (As a “rule of thumb”, Type I Ultrapure water contains dissolved solids at the level of “a few parts per billion”). 

 

Centrifuges

ThermoSavant Express speedvac concentrator
Location: Rm 5201
The speedvac (or vacuum centrifuge) is ideal for removing small quantities of solvent from multiple samples at once. It can accommodate a variety of vial sizes as well as standard microplates. Contact Keck Center TA for training.

Beckman Allegra 6KR bucket centrifuge
Location: Rm 5201
This centrifuge features a swinging bucket rotor that can accommodate 15 mL or 50 mL conical tubes, microcentrifuge tubes, and standard microplates. It is a refrigerated unit which can operate between -5 °C and 25 °C and has a max speed of 4,730 rpm (4,550 x g). Contact Keck Center TA for training.

Beckman-Coulter Avanti J-25I centrifuge
Location: 5th floor hallway (between Matthews - Shane Tower)
The centrifuge can accommodate much larger samples at higher speeds than the Allegra 6KR. Canisters and rotors are equipped with biosafety lids, so this centrifuge is ideal for pelleting cells or cell-debris. Two rotors are available: (1) JLA-10.5000: 4 x 500 mL samples, max speed 10,000 rpm (18,600 x g) or (2) JA-25.50: 8 x 50 mL samples, max speed 25,000 rpm (75,600 x g). A calendar is located next to the centrifuge to allow users to schedule usage in advance. Contact Keck Center TA for training.

Beckman L-70 Ultracentrifuge (Class R)
Location: 5th floor hallway (between Matthews - Shane Tower)
The ultracentrifuge can operate at much higher speeds and allows for the faster separation of samples. Some example applications include: sucrose gradient isolation of proteins, density gradient fractionation of lipoproteins, fractionating subcellular components (nucleosomes, microsomes, mitochondria, etc.), plasmid purification in CsCl, and RNA pelleting in CsCl. Two rotors are available: (1) Type 45 Ti: 6 x 94 mL samples, max speed 45,000 rpm (235,000 x g) or (2) Type 70 Ti: 8 x 39 mL samples, 70,000 rpm (504,000 x g). Contact Keck Center TA for training.

 

Microscopes

Nanoscope IV atomic force microscope (AFM)
Location: Subbasement Rm S340
Currently maintained by the Hamers and Jin groups. More details coming soon...

Olympus IX81 motorized inverted microscope
Location: Biochem addition, Rm 475
The Olympus IX81 microscope is a motorized inverted microscope that can be used for DIC/polarized light and fluorescence observations. It is equipped with a single mercury arc lamp that can be used to excite standard fluorophores such as DAPI, GFP, FITC, and TRITC. The motorized stage on this inverted scope can accommodate slides or multi-well dishes with four corners. Images are collected using a Hamamatsu digital camera and analyzed with analySIS software from Soft Imaging Systems. Contact Joe Klim, jrklim at wisc.edu for training.

Nikon Eclipse TS100 fluorescence microscope
Location: Dark room 5143, hallway (between Matthews - Shane Tower)
This inverted microscope is ideal for routine culture and lab tests. In addition to phase contrast, this model supports various microscopy techniques, including epi-fluorescence (useful in situations such as investigating GFP expression) and HMC. This microscope is equipped with a 2S2MBW monochromatic high speed CCD camera and operated through NIS Elements F imaging software. Contact Keck Center TA for training.

 

Freezers, incubators, and other facilities

Autoclaves
Location: 5th floor Daniels; subbasement
Two autoclaves are located within the department for the sterilization of equipment, growth media, and biohazardous waste. The newer autoclave is located on the 5th floor, with a hardier model found in the subbasement. The autoclave in the subbasement has a much longer cycle, and is recommended for use in sterilizing biohazardous waste.

Shaking incubator (37 °C)
Location: 5th floor hallway (between Matthews - Shane Tower)
This shaking incubator is kept at 37 °C, 250 rpm at all times. It has exchangeable restraints to accommodate culture tubes and Erlenmeyer flasks of various sizes.

Water jacketed CO2 incubator
Location: Rm 5207A
This static incubator is maintained at 37 °C, 5% CO2 injected for the growth of eukaryotic cell cultures.

Fisher Isotemp incubator
Location: Rm 5207A
This static incubator is available for culturing prokaryotic and eukaryotic samples. The temperature can be adjusted for the researcher's needs, and the incubator should be reserved in advance.

Fisher Isotemp oven
Location: Rm 5207A
This oven is maintained at 30 °C for pre-warming of media before inoculation.

Cold room (Rm 5145)
Location: Rm 5145, hallway (between Matthews - Shane Tower)
This walk-in cooler is maintained at 4 °C, and has four benches to allow for the storage of biological materials, or to accommodate reduced-temperature reactions.

Large-scale -78 °C freezer
Location: 5th floor hallway (between Matthews - Shane Tower)
Freezer for the storage of biological samples; all samples should be kept within boxes in racks, and each box should be labeled with Group – User.

Kenmore freezers -20 °C
Location: Rm 5201, Rm 5207A
Four dorm-size -20 °C freezers are distributed within the Keck Center. All materials being stored in these freezers should be labeled with Group – Name – Date.